Genetically Engineered Animal Models

Pronuclear Microinjection

Pronuclear microinjection produces transgenic mice that have one or more copies of the transgene integrated randomly in their genome. Purified DNA is microinjected into fertilized oocytes and survivors are transferred into pseudo-pregnant recipient female mice. TransARC transfers a minimum of 100 viable microinjected zygotes, which optimally produces a minimum of 20 pups for analysis (dependent on transgene). Within 2-3 weeks of birth, potential founder pups are ear notched and the tissue is delivered to the researcher for genotype analysis.

Due to the large variation in gene function, TransARC cannot guarantee that a transgene will produce transgenic mice or that animals carrying the transgene will possess the desired protein expression or transmit the transgene to the F1 generation.

    Embryonic Stem Cell Injection

Injection of embryonic stem cells (ESCs) into pre-implantation stage blastocysts is an efficient way to introduce gene mutations into the mouse’s genome. In contrast to pronuclear microinjection, ESC targeted mutagenesis can produce animals with a precise, targeted mutation of the gene of interest. This service includes microinjection of one ESC clone into 60-80 blastocysts, which are then transferred into pseudo-pregnant recipient female mice. Within 2-3 weeks of birth, pups are evaluated for coat colour chimerism.

Since ESCs can harbour mouse disease, TransARC requires that all cell lines entering their facilities are tested for pathogens prior to admittance. Currently, TransARC will accept ESCs from the following sources:

   1) In-house: If the TransARC facility has generated the mutant ESCs, additional pathogen testing will not be required as the facility    periodically tests parental cell lines

   2) Outside source: If the ESCs come from a source other than TransARC, they must be tested for pathogens prior to admittance into the    facilities.

Please contact the Transgenic Core (transarc@bcprc.ca) for more information.

 TransARC Service Request Form