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Rederivation

Rederivation is a safe, efficient method to transfer animals with a questionable health status into a specific pathogen free environment. TransARC has the ability to receive animals of various health backgrounds and perform rederivation by retrieval of pre-implantation of embryos into clean recipient females.

   Fresh Rederivation from Mice

This service is valuable for researchers who would like to obtain unique mouse strains that are only available from facilities that have pathogens. , which precludes their entry into most specific pathogen free facilities in BC. The researcher supplies TransARC with mutant mice and TransARC houses them within their quarantine facility. TransARC obtains pre-implantation embryos and transfers them into clean pseudopregnant females within the specific pathogen free barrier facilities. Rederived pups can be health tested at 6-8 weeks old and transferred to the researcher’s facility.


   Rederivation from Embryos

Many Networks, Consortiums and researchers are now able to provide collaborators with embryos, instead of live mice. The researcher supplies fresh or frozen embryos (a minimum of 30) to TransARC. TransARC prepares the embryos upon receipt and transfers them into clean pseudopregnant females within the specific pathogen free barrier facilities. Three weeks later, rederived pups are born. The rederived mice can be health tested at 6-8 weeks old and transferred to the researcher’s facility.


   In Vitro  Fertilization

In vitro  fertilization (IVF) is a useful service for researchers who are interested in obtaining a unique strain of mice that is only available as cryopreserved sperm. This service can also be used for strain rescue if only male mice still exist for the strain in question. The researcher supplies TransARC with male mice or sperm. TransARC harvests or prepares sperm and oocytes and performs IVF. Fertilized oocytes are transferred into clean pseudopregnant females within the specific pathogen free barrier facilities. IVF-derived pups can be health tested at 6-8 weeks old and transferred to the researcher’s facility. IVF-derived embryos can also be used for cryopreservation, if requested.


Please contact the Transgenic Core (transarc@bcprc.ca) for more information.

 TransARC Service Request Form