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Embryonic Stem Cell Manipulation

Gene Targeting

Embryonic stem cell (ESC) mutagenesis is a powerful tool for the introduction of gene mutations into the mouse’s genome. This procedure generates cells with a precise mutation, either knock-out or knock-in, of the gene of interest and can result in total loss of gene function, replacement of a gene or conditional expression of the mutation. TransARC electroporates the researcher’s DNA construct into in-house proven ESCs, then selects, picks, freezes replicas and prepares cell aliquots of 350-400 individual clones. Cell clones are delivered to the researcher for genotype analysis. Once the researcher has identified positive clones, these are thawed and expanded for either transfer back to the researcher, or injection into blastocysts for generation of a mouse model.


 

 

 

   Novel Embryonic Stem Cell Derivation

TransARC derives ESCs from novel mouse strains of the researcher’s choice. This service includes one attempt at producing ESCs utilizing a maximum of 5 mice. ESCs are derived from the inner cell mass of blastocyst stage pre-implantation embryo. Blastocysts are retrieved and plated to allow the inner cell mass to develop into an outgrowth. Outgrowths are then picked and transferred into 96-wells on mouse embryonic fibroblasts. After 14-28 days, ESCs are visible and are further expanded and frozen as aliquots for the researcher.


Please contact the Transgenic Core (transarc@bcprc.ca) for more information.

 TransARC Service Request Form